ADC Discovery Service

Service Highlights

• Diverse antibody discovery technical campaigns:
  

  Hybridoma, phage display, single B cell screening, fully human antibody discovery etc.
  Multiple technical routes in parallel to improve project success

• Multiple molecular types:
  

  Monoclonal antibody, bispecific monoclonal antibody, single domain antibody, antibody fragment

• High-throughput antibody screening strategy:
  

  ProBio conducts high-throughput internalization screening in the supernatant screening stage to identify antibody leads with desirable internalization capability in the early discovery stage.

  
• One stop antibody optimization solution:
  

  ProBio offers a comprehensive solution of ADC antibody optimization service based on functionality and developability, which includes antibody humanization, antibody affinity tuning, Fc engineering(ADCC,CDC, ADCP enhancement, Fc silence, half-life extension).

• Types of toxins available from ProBio (partial):

  Payload categories	Products
  Maytansinoid	DM1, DM2, DM3, DM4
  Auristatins	MMAE, MMAD,MMAF
  Eribulin derivatives	Eribulin Mesylate
  Tubulysins	Tubulysin D, Tubulysin M
  PBD Dimer	SG3199
  Doxorubicin	PNU-159682
  Camptothecin	Exatecan Mesylate
  Amanitin derivatives	α-Amanitin
  TLRs derivatives	Resiquimod
  STING derivatives	Cyclic-di-GMP

• ProBio offers a variety of ADC coupling modalities (Cysteine/Lysine based conjugation & site-specific conjugation) and evaluates conjugation quality based on the detection of DAR and ADC purity using a variety of methods including SDS-PAGE, HIC-HPLC, SEC-MS, RPLC-MS.

ProBio provides 7 different ADC bioassay methods for antibody internalization, cell cytotoxicity assays and bystander effect to meet the specific needs in ADC validation for our clients.

Scope	Internalization assay				Cytotoxicity assay	Bystander effect
Cell based assay	Live-cell imaging based Internalization	Toxin-conjugated mAb based cytotoxicity assay		pH-Indicator based Internalization	Temperature shift based Internalization	Cell growth inhibition assay	Medium transfer assay
General designation	IncuCyte	DT3C	FabFc-ZAP	pHrodo	Temperature Shift	Cytotoxicity assay	Bystander effect
Molecule	Naked Antibody					ADC or Toxin	ADC
Analyzer	Live-Cell Analyzer	Microplate Reader		FACS	FACS	Microplate Reader/ Live-Cell Analyzer	Microplate Reader
Feature	Real-time and kinetics analysis	High sensitivity High throughput Close to the cytotoxic MOA		Specific	Cost-effective Susceptible to multiple factors	High throughput	Simultaneous detection: Ag+ cell cytotoxicity and Ag- bystander effect
Application	Most versatile Best choice	Full range Cost-effective Alternative choice	High material cost	Client favor	For clients with limited budget	Full range	Bystander effect Potential security

80+ positive reference antibody dose-response curve (freely available)
Target cells 200+ tumor cell lines and 100+ overexpression cell lines

ProBio provides one-stop ADC in vivo pharmacology solution services including in vivo pharmacodynamic evaluation based on animals models, in vivo pharmacokinetics, preclinical toxicology studies, and biomarker assay, which are meet the compliance requirements for ADC declarations and assist you in faster, superior, and more provincial ADC development.

• Extensive model resource

  • Abundance of mouse strains
  • 6 tumor bearing animal models
  • 80+ Tumor cell models
  • 200+ Tumor cell banks
  • 24 Tumor indications

• Pharmacokinetic experiments

  • Method development and validation
  • Pharmacokinetic experiments in cynomolgus monkeys and other primates
  • Tissue distribution experiment
  • Receptor mass experiments

• Toxicological studies

  • Toxicity with a single administration
  • Dose exploration experiments
  • Long-term toxicity testing
  • Local toxicity
  • Tissue cross-reactivity experiments