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ProSpeed™ Single B Cell Antibody Discovery Service

Integration of the ProSpeed^TM Expression with Beacon® Platform

Fast and Cost-effective Discovery of Functional Ab Leads in 1 Month!

Single B cell screening platform conducts isolation, screening, sorting, and evaluation on the B cells, avoiding the cell fusion and the library construction step, saving time from months to weeks. ProBio has upgraded the ProSpeed™ Single B Cell Antibody Discovery Platform, and integrated Berkeley Lights‘s Beacon® technology with our proprietary ProSpeed^TM expression. It is one of the disruptive, best-in-industry platforms for single B-cell screening, enabling customers to achieve fast and cost-effective discovery of confirmed functional antibody leads in as fast as 1 month.

A Perfect Combination with ProSpeed^TM
Expression and Beacon® Platform

Integration of the ProSpeed expression with Beacon® platform to allow fast and cost-effective discovery of functional Ab leads
Breaking-through the technical limitations of Beacon® platform

Expedited Timeline

Multiple sequential screening completed in 1 day
As fast as 1 month to get functional sequences

Extensive Experience

Introduced Beacon® platform since 2019
Delivery of over 110 projects
Extensive experience in various targets

High Diversity

Functional screening at early phase for largest B cell pool possible
Less risk in diversity loss

Download the poster to discover how we integrated a ProSpeed^TM expression system to the Beacon® platform

ProSpeed™ Single B Cell Antibody Discovery Platform

ProBio‘s ProSpeed™ Single B Cell Antibody Discovery Platform is based on Beacon®, which combines optical positioning with microfluidic technology. It enables precise and efficient sorting of single cells into nano-pen chambers, completing diverse and robust assays within minutes. The desired clones were exported into 96 well plates for downstream HTP ProSpeed^TM expression, further functional screening assay, and sequencing.

This single B cell screening technology maximizes our access to antibody repertoires, making it inherently superior in preserving B cell diversity, and the validated functional antibody sequences will be obtained as fast as 1 month.

ProSpeed^TM Expression

The perfect combination with Beacon® platform

The challenges in Beacon screening:

To address these limits effectively, we integrate the ProSpeed^TM expression with Beacon® platform to allow fast and cost-effective discovery of functional Ab leads.

learn how we utilize ProSpeed^TM expression in single B cell screening

Case Study: anti-DLL3 antibody Discovery

Up to 4 sequential assays were included in the screening workflow

Human DLL3	98 binders
Cyno DLL3	127 binders
Mouse DLL3	58 binders
Human DLL3 cell	50+ binders

Exported Cross	Unique Sequence
61 out of 100	21 out of 23

Phylogenetic Tree

Sequences from Single B screening are highly diverse.
There are overlapping germline families from both platforms, but also unique families from Single B or hybridoma.