pH-selective Antibody Discovery Service

• Obtain more pH-selective Abs leads in the early R&D stage

• 2 antibody engineering approaches to increase Ab’s pH selectivity

The pH-selective antibody is designed to exhibit varying binding affinities depending on the pH level in different environments. This unique property is beneficial in therapeutic applications where precise targeting and controlled release of drugs are essential.

ProBio provides a one-stop solution for pH-selective antibody discovery. Our services enable the identification of high-potential pH-selective Ab leads through customized screening strategies in the early discovery stage. Additionally, we offer two featured antibody engineering approaches to further enhance the antibody's pH sensitivity. This two-pronged approach significantly increases the possibility of obtaining satisfied pH-selective antibodies.

One-stop solution for pH-selective antibody discovery

• Multi Lead Generation Technology Platforms
  Mice/Alpaca Immunized Library

  Alpaca Naïve Library

  Human Naïve Library

  pH selective panning/screening to enrich hits with desired binding profile under different pH conditions

• Two Antibody Engineering Approaches

  Deluxe Package

  More comprehensive screening strategy

  • Construct 2 rounds of Trimer combinatorial mutagenesis library
  • HTP transient expression and confirmation

  Express Package

  Faster and more cost-effective

  • Full CDR histidine scanning
  • HTP transient expression and confirmation

Case Study：pH-selective Ab Discovery via Alpaca Naïve and Immunized Library

Project objective: to discover Ab sequences de novo with preferential binding to target at pH 6.0 than pH7.4 via alpaca naïve library

Project objective: to discover Ab sequences de novo with preferential binding to target at pH 6.0 than pH7.4 via alpaca immunized library

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