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Beacon-based sdAb Discovery

Although library-based sdAb discovery technology is well-developed, some limitations remain, such as sequence diversity loss, difficulties in conducting functional assays, and the considerable time required.

To overcome these challenges, ProBio has integrated the proprietary alpaca single B cell preparation technology, the advanced Beacon® single B cell screening platform and ProSpeed™ antibody expression platform. This novel approach allows you to obtain more functional sdAb binders with higher sequence diversity in as fast as 9 weeks.

Learn More About SPSSdAb™ Screening Platform

Library-based sdAb Discovery

ProBio offers two classic library-based sdAb discovery services: sdAb naïve library and immunized library. We combine these high-quality libraries with customized phage display panning strategies and our proprietary SPSSdAb™ screening platform to help you discover high-potential sdAb antibody leads.

  • sdAb Naïve Library

    • 300 Alpaca donors

    • Library size: 2×1011 (expanding)

    • In frame rate/ORF rate: >95%

  • sdAb Immunized Library

    • Easy access to Alpaca farms

    • Using only naïve Alpaca for each project

Feel free to download the poster to discover how we create antibody leads for CAR-T therapy.

Feel free to download the poster to discover how we create antibody leads for CAR-T therapy via our sdAb library.

Service Packages

Milestone Service Timeline Deliverable
1 Standard immunization 6-8 weeks
  • Antibody sequences
  • 0.5 mg/Ab purified antibodies (Optional)
2 Single B cell preparation and screening (2 chips) 1 week
3 Prospeed™ expression (optional) 1-2weeks
4 Screening Assays (optional)
5 V-region sequencing 1-2 weeks
6 Recombinant antibody production/confirmation(optional) 3-4 weeks
Total (not including optional) 8-12 weeks
Milestone Items Time (week) Deliverable
1 Llama/Alpaca immunization 6-8 weeks Delivery: Up to 10 sequences
0.5 mg/Ab purified antibodies (Optional)
2 sdAb phage display library construction(size of > 5 x 10^8) 2-3 weeks
3-1 Phage display panning 1-2 weeks
3-2 ELISA or FACS Screening 1-2 weeks
4 High throughput screening (ligand blocking screening/Affinity ranking) (optional) 1-3 weeks
5 sdAb expression & purification (optional) 3-4 weeks
6 Total (no optional steps included) 11-18
Milestone Items Time (week) Deliverable
1-1 Phage display panning 1-2 weeks Delivery: Up to 10 sequences
0.5 mg/Ab purified antibodies (Optional)
1-2 ELISA or FACS Screening 1 weeks
2 sdAb expression & purification & sequence delivery (Up to 10 sequences) 3-4 weeks
3 Additional sequence delivery (optional) /
Total (no optional steps included) 2-3 weeks

One-stop Solution From Target to Preclinical Candidates

Beacon-based sdAb Discovery
sdAb Immunized
Library
sdAb Naïve Library
In Vitro Functional Assay
sdAb Lead Optimization
In Vivo Pharmacology
Developability Assessment